The backup could be used to create healthy koala embryos through artificial insemination and in vitro fertilization (IVF), the researchers said in a statement. When koalas (Phascolarctos cinereus) die, unique traits and diverse genes that may help them adapt to changing environments are lost, but the new project offers a way to store this valuable material, the team said.
Australia is facing a paradox with its koalas. In some regions of Queensland and New South Wales, koala populations have crashed by as much as 80% since the late 1990s due to deforestation, bushfires, drought and disease. This prompted the Australian government to change koalas' conservation status in their eastern range from "vulnerable" to "endangered" in 2022.
To protect against koalas' decline, the scientists will freeze koala reproductive cells in liquid nitrogen (LN2), which has a boiling point of minus 321 degrees Fahrenheit (minus 196 degrees Celsius). This method would enable the team to cryopreserve the cells for several decades until they are needed, said Vincent Lynch, an evolutionary developmental biologist and associate professor of biological sciences at the University at Buffalo in New York.
Andres Gambini, reproductive biologist at the University of Queensland
The sperm and eggs will be supplied by wildlife hospitals that will harvest sex cells from dead koalas or koalas that can no longer breed due to disease or trauma, according to the statement.
The researchers will then test the cells for Chlamydia pecorum, a highly contagious and deadly form of chlamydia. In koalas, this infection causes painful urinary tract infections, gastrointestinal issues, and conjunctivitis, which can lead to blindness. It can also cause infertility in females. C. pecorum is one of the main contributors to koalas' decline in recent years, with infertility driving a sharp reduction in the number of koala joeys being born. In the worst-affected populations, situated mostly in Queensland and New South Wales, almost 90% of koalas are infected with chlamydia.
Andres Gambini and doctoral student Patricio Dandy Palacios, who participated in the project, want to preserve koala sex cells in liquid nitrogen. (Image credit: The University of Queensland)
In 1998, Johnston was part of a research team at the University of Queensland that created the world's first koala joey born through artificial insemination. The new project also builds on a 2025 study, led by Gambini, that produced the first-ever IVF kangaroo embryos. (These did not result in live births; at the time, the scientists said that would take another decade.)
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Although conservationists are concerned about the fast rate of koalas' decline, "there is a chance" that scientists can save the species through cryopreservation, Lynch said.
"I support multipronged approaches like this," he said. "By preserving the environment with traditional conservation we allow re-introductions because the species have somewhere to live."
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