Excitation spectral microscopy integrates multi-target imaging and quantitative biosensing ...Middle East

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The multiplexing capability of fluorescence microscopy provides valuable means for interrogating complex intracellular dynamics. Scientists from UC-Berkeley demonstrated that excitation spectral microscopy can integrate multi-target imaging of up to 6 subcellular structures with quantitative imaging of fluorescent biosensors in live cells. Among other results, they elucidated the evolution of the mitochondrial matrix pH in the complex, Parkin-mediated mitophagy pathway. This new spectral imaging scheme provides exceptional opportunities for highly multiplexed, quantitative fluorescence microscopy.

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